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Charde, Manoj S.
- Evaluation of Wound Healing, Anti-Inflammatory and Antioxidant Activity of Rhizomes of Curcuma longa
Authors
1 J. L. Chaturvedi College of Pharmacy, Electronic Zone Building, MIDC, Hingna Road, Nagpur-440016, Maharashtra, IN
2 Kamla Nehru College of Pharmacy, Wardha Road, Buttibori, Nagpur, MS, IN
3 Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur-440033, MS, IN
Source
Research Journal of Pharmacology and Pharmacodynamics, Vol 2, No 1 (2010), Pagination: 42-47Abstract
The present study deals with evaluation of antioxidant, wound healing and anti-inflammatory activity of ethanolic extract of Curcuma longa Linn. rhizomes. The ethanolic extract prepared by maceration technique was subjected to screen for antioxidant activity using DPPH radical scavenging method and wound healing activity using incision, excision, histopathological and dead space wound model and the study was supported with evaluation of granuloma tissue to estimate hydroxyproline content and histopathological evaluation. The anti-inflammatory study was carried out by using carageenan induced rat paw odema method. The tested extract of different dilutions in range 200 μg/ml to 1000 μg/ml shows activity in range of 9.34% to 18.55%. Significant increase in wound closure rate, skin breaking strength, granuloma breaking strength was observed. The hydroxyproline content was also increased with decrease in scar area. The initial healing action might be due to increased collagen deposition and better alignment, with the obtained results it can be concluded that Curcuma longa extract has significant wound healing activity and initial healing may be due to presence of curcuminoids and antimicrobial agents. The extract shows prominent anti-inflammatory activity as compared to that of standard (Ibuprofen gel). The extract shows good anti-inflammatory activity on carageenan induced rat paw odema method.Keywords
Antioxidant, Wound Healing, Anti-Inflammatory, Curcuma longa.References
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- RP-HPLC Method for Simultaneous Estimation of Calcipotriene and Betamethasone Dipropionte and Its Combined Dosage Form
Authors
1 NRI Institute of Pharmacy, Bhopal, IN
Source
Asian Journal of Research in Chemistry, Vol 4, No 4 (2011), Pagination: 602-606Abstract
This work is concerned with application of simple, accurate, precise and highly selective reverse phase high performance liquid chromatographic (RP-HPLC) method for simultaneous estimation of Calcipotriene and Betamethasone dipropionate in combined dosage form. Chromatographic separation was achieved isocratically at 25oC on Zorbax SB-300 C18 column (150 _ 4.6 mm) with a mobile phase composed of Methanol: 0.005M (NH4)2PO4 (pH 7.4)- Buffer in the ratio of 45:55 % v/v/v at flow rate of 1.0 ml/min. Detection is carried out using a UV detector at 264 nm. The retention time of Calcipotriene and Betamethasone dipropionate was found to be 7.99 min and 13.99 min. respectively. The method was found to be linear in the range of 0.8-1.4 _ppm with mean recovery of 101.16%+ 0.6 % for Calcipotriene and 100.29%+0.25 % for Betamethasone dipropionate. The correlation coefficients for all components are close to 1. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. Thus the proposed method was successfully applied for simultaneous determination of Calcipotriene and Betamethasone dipropionate in routine analysis.
Keywords
Calcipotriene, Betamethasone Dipropionate, RP-HPLC.- Enhancement of Dissolution Profile of Poorly Water Soluble Drug Using Water Soluble Carriers
Authors
1 Assistant Professor, Dr. Rajendra Gode Institute of Pharmacy, Amravati 444602, Maharashtra Principal, Dr. Rajendra Gode Institute of Pharmacy, Amravati 444602, Maharashtra, IN
2 Assistant Professor, Government College of Pharmacy, Karad 415124, Maharashtra, IN
Source
Asian Journal of Pharmaceutical Research, Vol 12, No 2 (2022), Pagination: 137 - 142Abstract
Teneligliptin Hydrobromide is a long-acting, orally bioavailable, pyrolidone anti-diabetic activity with a solubility of 1.7mg/ml in water which also depends upon the pH and temperature of the solvent. So, Solid dispersion of drug with different polymers an attempt was made to improve dissolution of teneligliptin hydrobromide. The aim of this study was to prepare, characterize and compare solid dispersions of poorly water soluble anti diabetic drug by using PVP and HPMC for enhancing the dissolution rate of the drug. The solid dispersions were prepared by physical mixing method and kneading method at 1:1, 1:2 and 2:1 ratios of drug to polymer. The drug-excipient interaction study showed that the drug and polymers were compatible with each other. The formulations were evaluated for percent drug content, micromeritics and in-vitro dissolution studies. In the present study it was seen that there was an increase in in-vitro drug release for solid dispersion as compared to the pure drug taken alone. Based on the pattern of drug release, the kneading method showed more drug release as compared to physical mix method. In physical mix method, the rate of dissolution of teneligliptin hydrobromide was increased in teneligliptin and Polyvinylpyrrolidone (PVP) with the proportion of (1:2) when compared to the other formulations. In kneading method, the rate of dissolution of teneligliptin hydrobromide was increased in drug and Hydroxypropylmethylcellulose (HPMC) with the proportion of (1:2) when compared to the other formulations. Finally, solid dispersion containing HPMC, as a carrier, gave faster dissolution rates among all the formulations and was selected as the optimized formulation inthis study.
Keywords
Teneligliptin Hydrobromide, Antidiabetic, Dissolution enhancement, Solid Dispersion, HPMC, PVPReferences
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